2019-01-30 09:03:482025-04-04 08:17:00
locus
BSU29090
BSU_29090
outlinks
bsu
BSU29090
BSU_29090
Gene
Phenotypes of a mutant
strongly reduced stationary phase mutagenesis [Pubmed|27399782]
a [[gene|polA]] [[gene|exoR]] double mutant is not viable (synthetic lethality) [pubmed|17114254]
strongly reduced stationary phase mutagenesis [Pubmed|27399782]
The protein
Catalyzed reaction/ biological activity
has DNA polymerase and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity [Pubmed|16045613]
maturation of Okazaki fragments (together with [[protein|RnhC|RNase HIII]]) [pubmed|30670546]
has DNA polymerase and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity [Pubmed|16045613]
maturation of Okazaki fragments (together with [[protein|RnhC|RNase HIII]]) [pubmed|30670546]
2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
The protein
Protein family
DNA polymerase type-A family (according to Swiss-Prot)
DNA polymerase type-A family (single member, according to UniProt)
The protein
Structure
[PDB|1XWL] (large fragment, corresponds to AA 310 to 880, Geobacillus stearothermophilus)
[PDB|6VDE] (from Mycobacterium smegmatis, 40.5% identity) [pubmed|32034423]
[PDB|1XWL] (large fragment, corresponds to AA 310 to 880, Geobacillus stearothermophilus)
Biological materials
Mutant
BKE29090 ([[gene|polA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA
BKK29090 ([[gene|polA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA
GP3532 (Δ[[gene|polA]]::spec), available in [SW|Jörg Stülke]'s lab
BKE29090 ([[gene|polA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA
BKK29090 ([[gene|polA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA
References
The protein
[SW|Domains]
5'-3' exonuclease domain (aa 174-268) (according to UniProt)
3'-5' exonuclease domain (aa 302-470) (according to UniProt)
The protein
[SW|Localization]
recruited to replication forks after induction of DNA damage [pubmed|31251806]